Modification:Article Title: Fractional ethanol precipitation modulates the structure and function of Coprinus comatus mycelial polysaccharides: alleviation of DSS-induced injury in Caco-2 cells via regulation of mitochondrial function
Article Snippet: .. Coprinus comatus strain (Xuzhou, Jiangsu, China); Caco-2 cells (Zibin Bio, Wuhan, China); Potato Dextrose Agar (PDA) (Hope Bio, Qingdao, China); Glucose (Zhiyuan, Tianjin, China); 1,1-Diphenyl-2-picrylhydrazyl (DPPH) (Macklin, Shanghai, China); L-Ascorbic acid (Vc) (Macklin); Trifluoroacetic acid (TFA) (ANPEL, Shanghai, China); Methanol (ANPEL); Sodium hydroxide (NaOH) (Sigma-Aldrich); Sodium acetate (Sigma-Aldrich); 2, 2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) (Macklin); Absolute ethanol (Fuyu Reagent, China); Hydrogen peroxide (H2O2); Ferrous sulfate heptahydrate (FeSO4·7H2O) (Shanghai Hushi, China); Dulbecco’s Modified Eagle Medium (DMEM) (Gibco); Fetal Bovine Serum (FBS) (Gibco); 1% Penicillin–Streptomycin solution; CCK-8 assay kit (Beyotime, China); Reactive Oxygen Species (ROS) assay kit (Beyotime); Superoxide Dismutase (SOD) assay kit (Beyotime); Mitochondrial ROS (mtROS) assay kit (Solarbio, China); JC-1 Mitochondrial Membrane Potential Assay Kit (Solarbio); Interleukin-6 (IL-6) ELISA Kit (Solarbio); Tumor Necrosis Factor-alpha (TNF- α ) ELISA Kit (Solarbio). ..
CCK-8 Assay:Article Title: Fractional ethanol precipitation modulates the structure and function of Coprinus comatus mycelial polysaccharides: alleviation of DSS-induced injury in Caco-2 cells via regulation of mitochondrial function
Article Snippet: .. Coprinus comatus strain (Xuzhou, Jiangsu, China); Caco-2 cells (Zibin Bio, Wuhan, China); Potato Dextrose Agar (PDA) (Hope Bio, Qingdao, China); Glucose (Zhiyuan, Tianjin, China); 1,1-Diphenyl-2-picrylhydrazyl (DPPH) (Macklin, Shanghai, China); L-Ascorbic acid (Vc) (Macklin); Trifluoroacetic acid (TFA) (ANPEL, Shanghai, China); Methanol (ANPEL); Sodium hydroxide (NaOH) (Sigma-Aldrich); Sodium acetate (Sigma-Aldrich); 2, 2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) (Macklin); Absolute ethanol (Fuyu Reagent, China); Hydrogen peroxide (H2O2); Ferrous sulfate heptahydrate (FeSO4·7H2O) (Shanghai Hushi, China); Dulbecco’s Modified Eagle Medium (DMEM) (Gibco); Fetal Bovine Serum (FBS) (Gibco); 1% Penicillin–Streptomycin solution; CCK-8 assay kit (Beyotime, China); Reactive Oxygen Species (ROS) assay kit (Beyotime); Superoxide Dismutase (SOD) assay kit (Beyotime); Mitochondrial ROS (mtROS) assay kit (Solarbio, China); JC-1 Mitochondrial Membrane Potential Assay Kit (Solarbio); Interleukin-6 (IL-6) ELISA Kit (Solarbio); Tumor Necrosis Factor-alpha (TNF- α ) ELISA Kit (Solarbio). ..
ROS Assay:Article Title: Fractional ethanol precipitation modulates the structure and function of Coprinus comatus mycelial polysaccharides: alleviation of DSS-induced injury in Caco-2 cells via regulation of mitochondrial function
Article Snippet: .. Coprinus comatus strain (Xuzhou, Jiangsu, China); Caco-2 cells (Zibin Bio, Wuhan, China); Potato Dextrose Agar (PDA) (Hope Bio, Qingdao, China); Glucose (Zhiyuan, Tianjin, China); 1,1-Diphenyl-2-picrylhydrazyl (DPPH) (Macklin, Shanghai, China); L-Ascorbic acid (Vc) (Macklin); Trifluoroacetic acid (TFA) (ANPEL, Shanghai, China); Methanol (ANPEL); Sodium hydroxide (NaOH) (Sigma-Aldrich); Sodium acetate (Sigma-Aldrich); 2, 2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) (Macklin); Absolute ethanol (Fuyu Reagent, China); Hydrogen peroxide (H2O2); Ferrous sulfate heptahydrate (FeSO4·7H2O) (Shanghai Hushi, China); Dulbecco’s Modified Eagle Medium (DMEM) (Gibco); Fetal Bovine Serum (FBS) (Gibco); 1% Penicillin–Streptomycin solution; CCK-8 assay kit (Beyotime, China); Reactive Oxygen Species (ROS) assay kit (Beyotime); Superoxide Dismutase (SOD) assay kit (Beyotime); Mitochondrial ROS (mtROS) assay kit (Solarbio, China); JC-1 Mitochondrial Membrane Potential Assay Kit (Solarbio); Interleukin-6 (IL-6) ELISA Kit (Solarbio); Tumor Necrosis Factor-alpha (TNF- α ) ELISA Kit (Solarbio). ..
Membrane:Article Title: Fractional ethanol precipitation modulates the structure and function of Coprinus comatus mycelial polysaccharides: alleviation of DSS-induced injury in Caco-2 cells via regulation of mitochondrial function
Article Snippet: .. Coprinus comatus strain (Xuzhou, Jiangsu, China); Caco-2 cells (Zibin Bio, Wuhan, China); Potato Dextrose Agar (PDA) (Hope Bio, Qingdao, China); Glucose (Zhiyuan, Tianjin, China); 1,1-Diphenyl-2-picrylhydrazyl (DPPH) (Macklin, Shanghai, China); L-Ascorbic acid (Vc) (Macklin); Trifluoroacetic acid (TFA) (ANPEL, Shanghai, China); Methanol (ANPEL); Sodium hydroxide (NaOH) (Sigma-Aldrich); Sodium acetate (Sigma-Aldrich); 2, 2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) (Macklin); Absolute ethanol (Fuyu Reagent, China); Hydrogen peroxide (H2O2); Ferrous sulfate heptahydrate (FeSO4·7H2O) (Shanghai Hushi, China); Dulbecco’s Modified Eagle Medium (DMEM) (Gibco); Fetal Bovine Serum (FBS) (Gibco); 1% Penicillin–Streptomycin solution; CCK-8 assay kit (Beyotime, China); Reactive Oxygen Species (ROS) assay kit (Beyotime); Superoxide Dismutase (SOD) assay kit (Beyotime); Mitochondrial ROS (mtROS) assay kit (Solarbio, China); JC-1 Mitochondrial Membrane Potential Assay Kit (Solarbio); Interleukin-6 (IL-6) ELISA Kit (Solarbio); Tumor Necrosis Factor-alpha (TNF- α ) ELISA Kit (Solarbio). ..
Enzyme-linked Immunosorbent Assay:Article Title: Fractional ethanol precipitation modulates the structure and function of Coprinus comatus mycelial polysaccharides: alleviation of DSS-induced injury in Caco-2 cells via regulation of mitochondrial function
Article Snippet: .. Coprinus comatus strain (Xuzhou, Jiangsu, China); Caco-2 cells (Zibin Bio, Wuhan, China); Potato Dextrose Agar (PDA) (Hope Bio, Qingdao, China); Glucose (Zhiyuan, Tianjin, China); 1,1-Diphenyl-2-picrylhydrazyl (DPPH) (Macklin, Shanghai, China); L-Ascorbic acid (Vc) (Macklin); Trifluoroacetic acid (TFA) (ANPEL, Shanghai, China); Methanol (ANPEL); Sodium hydroxide (NaOH) (Sigma-Aldrich); Sodium acetate (Sigma-Aldrich); 2, 2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) (Macklin); Absolute ethanol (Fuyu Reagent, China); Hydrogen peroxide (H2O2); Ferrous sulfate heptahydrate (FeSO4·7H2O) (Shanghai Hushi, China); Dulbecco’s Modified Eagle Medium (DMEM) (Gibco); Fetal Bovine Serum (FBS) (Gibco); 1% Penicillin–Streptomycin solution; CCK-8 assay kit (Beyotime, China); Reactive Oxygen Species (ROS) assay kit (Beyotime); Superoxide Dismutase (SOD) assay kit (Beyotime); Mitochondrial ROS (mtROS) assay kit (Solarbio, China); JC-1 Mitochondrial Membrane Potential Assay Kit (Solarbio); Interleukin-6 (IL-6) ELISA Kit (Solarbio); Tumor Necrosis Factor-alpha (TNF- α ) ELISA Kit (Solarbio). ..
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